Use this url to cite publication: https://hdl.handle.net/20.500.12512/22523
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Comparative study of the influence of method on the quantity of proteins extracted from the raw material of (Baptisia australis (L.) Willd) roots / Gustė Vitkutė, Nijolė Savickienė, Ona Ragažinskienė, Gabrielė Balčiūnaitė
Type of publication
Tezės kitame recenzuojamame leidinyje / Theses in other peer-reviewed publication (T1e)
Title
Comparative study of the influence of method on the quantity of proteins extracted from the raw material of (Baptisia australis (L.) Willd) roots / Gustė Vitkutė, Nijolė Savickienė, Ona Ragažinskienė, Gabrielė Balčiūnaitė
Publisher (trusted)
Lithuanian University of Health Sciences |
Date Issued
Date Issued |
---|
2019-05-03 |
Extent
p. 11-11, no. US-01.
Is part of
International conference „Contemporary pharmacy: issues, challenges and expectation“ : abstract book : May 3rd, 2019 Kaunas, Lithuania / Lithuanian University of Health Sciences, Faculty of Pharmacy, Department of Drug Technology and Social Pharmacy ; Edited by: Inga Matulytė. Kaunas : Lithuanian University of Health Sciences, 2019. ISBN 9789955156000.
Version
Originalus / Original
Series/Report no.
Summaries of oral presentations.
Description
ISBN 978-9955-15-600-0.
Bibliogr.: p. 11
Field of Science
Abstract
Baptisia australis (L.) Willd. - perennial plant of the Fabaceae family, which preparations have anti-inflammatory, analgesic, antioxidant effects and can be used for cardiovascular prophylaxis. It has been investigated that Baptisia australis (L.) Willd. herbal raw material, such as grass, roots, accumulates flavonoids, alkaloids, triterpene saponins and other compounds, but proteins were not quantified. In this research, protein concentration was evaluated based on the protein extraction method [1]. The aim of the experiment: To determine the influence of protein extraction from Baptisia australis (L.) Willd. roots methods on the quantitative composition of proteins. Experimental tasks: 1. To determine the amount of proteins extracted from the roots of Baptisia australis (L.) Willd. raw material using PBS (phosphate buffer solution) and precipitating proteins with ammonium sulfate. 2. To determine the amount of proteins extracted from the roots of Baptisia australis (L.) Willd. raw material using PBS and 0.5% thiourea solution and precipitating proteins with ammonium sulfate. 3. To compare the effect of protein extraction methods based on protein content. Material and methods: Material – fresh Baptisia australis (L.) Willd. roots were collected in Vytautas Magnus University botanical garden medical herbs section. For the first method of protein extraction from raw material we used a phosphate buffer solution (PBSx10) prepared from Na2HPO4, NaH2PO4, NaCl and deionized water; For the second method of the protein extraction from raw material we used phosphate buffer solution (PBSx10) adding 0.5% thiourea solution. For both methods we used protein precipitation method by ammonium sulfate, resuspension method by PBSx1 [2], spectrophotometric (at 595 nm) protein determination by Bradford method [3], statistical analysis method based on SPSS 17.0 and Microsoft Excel. Results: Extraction of the raw material by the first method showed that 1,056 mg ± 0,170mg[...
Type of document
type::text::conference output::conference proceedings::conference paper
ISBN (of the container)
9789955156000
Other Identifier(s)
(LSMU ALMA)990000983740107106
Coverage Spatial
Lietuva / Lithuania (LT)
Language
Anglų / English (en)
Bibliographic Details
3