Development and evaluation of test for identification of lysozyme hydrochloride in the composition of medicated chewing gums with ascorbic acid
| Author | Affiliation | |
|---|---|---|
National University of Pharmacy, Kharkiv, Ukraine | ||
Yevtifieieva, Olha | Department of Pharmaceutical Chemistry, National University of Pharmacy, Kharkiv, Ukraine | |
| Date |
|---|
2022-10-21 |
Plenary Session. Abstracts. Scientists session
ISBN 978-9955-15-769-4
Background: The combination of lysozyme hydrochloride (LH) and ascorbic acid (AsA) in the medicated chewing gum (MCG) is an effective method of prevention and treatment of dental diseases, in particular inflammatory diseases of the periodontium (gingivitis, periodontitis), mucous membranes (stomatitis), caries and manifestations of xerostomia. The development of methods for quality control of lysozyme hydrochloride in the MCGs is quite relevant. Aim: The aim of the study is to develop a spectrophotometric method for the identification of lysozyme hydrochloride by the method of specific absorbance in the composition of MCGs with ascorbic acid. Results: The results of the study of absorption spectrum LH in acetate buffer solution (pH 5.4) and phosphate buffers solutions pH 6.0; pH 6.2 and pH 6.8 (1 in 10000) prove that the optical absorption of LH is most stable in phosphate buffer solutions pH 6.0 and pH 6.2. The absorption maximum at 281 nm was selected for analysis in phosphate buffer solutions pH 6.2. AsA is very sensitive to changes in pH. AsA is stable in acidic pH 3.0. In a neutral medium, the maximum absorption of AsA is shifted to 265 nm. Determination of LH was performed after preliminary oxidation of AsA with ammonia solution of silver nitrate. The specific absorption rate in the range of application of the method from 80.0% to 120.0%, n = 9 is determined. The average value of the specific absorbance is A_1sm^(1%)= 25.40 ± 0.49, RSD = 0.84%, ∆As = 1.94%, n = 9. The specific absorption at the absorption maximum from 24.90 to 25.90 is proposed. Conclusion: An easy, sensitive and inexpensive spectrophotometric method for the determination of LH in the composition of MCG with ascorbic acid has been developed. Accuracy (25.40 ± 1.94%) and precision were found to be satisfactory. The proposed technique has practical value for carrying out routine quality control of MCG.